write all the steps to prepare temporary Mount of onion peel.
Answers
Answer: Steps for preparation of onion peel temporary mount:
1. In a petridish pour distilled water.
2. Pull out peel from onion by using needle and forceps.
3. Put the peel in petridish containing water.
4. Pour few drops of safranin solution in the watch glass.
5. With the help of brush, transfer the peel to the watch glass containing safranin solution.
6. Peel should remain in the safranin solution for 30 seconds.
7. Take out the peel from safranin solution by using brush and place it in watch glass containing distilled water.
8. Pour few drops of glycerine on a dry glass slide.
9. With the help of brush, place the peel onto the slide containing glycerine.
10. Put cover slip on the peel gently to avoid air bubbles.
11. Observe the slide under the microscope.
Initially we have to cut the onion into two half's. Then cut a small part from an inner scale leaf with the help of a blade. Then separate a thin, transparent peel from the convex surface of the scale leaf with the help of forceps. Then keep this peel in water and stain with safranin stain. Observe the peel under a microscope. This is how the experiment should be done.