write all the steps to prepare temporary mount of onion peel
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● Pour some distilled water into a watch glass.
● Peel off a leaf from half a piece of onion and using the forceps, pull out a piece of transparent onion peel (epidermis) from the leaf.
● Put the epidermis in the watch glass containing distilled water.
● Take a few drops of safranin solution in a dropper and transfer this into another watch glass.
● Using a brush, transfer the peel into the watch glass containing the safranin solution.
● Let this remain in the Safranin solution for 30 seconds, so that the peel is stained.
● Take the peel from the Safranin solution using the brush and place it in the watch glass containing the distilled water.
● Take a few drops of glycerine in a dropper and pour 2-3 drops at the center of a dry glass slide.
● Using the brush, place the peel onto the slide containing glycerine.
● Take a cover slip and place it gently on the peel with the aid of a needle.
● Remove the extra glycerine using a piece of blotting paper.
● Place this glass side on the stage of the compound microscope and view it.
● Peel off a leaf from half a piece of onion and using the forceps, pull out a piece of transparent onion peel (epidermis) from the leaf.
● Put the epidermis in the watch glass containing distilled water.
● Take a few drops of safranin solution in a dropper and transfer this into another watch glass.
● Using a brush, transfer the peel into the watch glass containing the safranin solution.
● Let this remain in the Safranin solution for 30 seconds, so that the peel is stained.
● Take the peel from the Safranin solution using the brush and place it in the watch glass containing the distilled water.
● Take a few drops of glycerine in a dropper and pour 2-3 drops at the center of a dry glass slide.
● Using the brush, place the peel onto the slide containing glycerine.
● Take a cover slip and place it gently on the peel with the aid of a needle.
● Remove the extra glycerine using a piece of blotting paper.
● Place this glass side on the stage of the compound microscope and view it.
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