write an account on collection of hemotoligical specimen and staining procedures in hematology .
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Answer:
Sample collection
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Blood for hematologic testing must be collected into an anticoagulant, preferably EDTA (purple top tube). Blood smears should be made at the time of collection to minimize storage-associated changes, which invariably occur. Venipuncture should be minimally traumatic to minimize platelet activation and should be done using a minimum of a 23G needle (21-22G is ideal for small animals and 18G is ideal for large animals) and 3-5 mL syringe (depending on the desired volume). Vacutainers can be used for sample collection, as can short butterfly catheters. For very small or pediatric patients, samples can be placed into microtainer tubes. Regardless of the tube used for sample collection, the blood must be adequately mixed with the anticoagulant during and after collection to prevent clotting. This is done by gentle inversion of the tube (tubes should never be shaken).
Answer:
Answer:
Sample collection
Share on FacebookTweet about this on TwitterShare on LinkedInPin on PinterestShare on StumbleUpon
Blood for hematologic testing must be collected into an anticoagulant, preferably EDTA (purple top tube). Blood smears should be made at the time of collection to minimize storage-associated changes, which invariably occur. Venipuncture should be minimally traumatic to minimize platelet activation and should be done using a minimum of a 23G needle (21-22G is ideal for small animals and 18G is ideal for large animals) and 3-5 mL syringe (depending on the desired volume). Vacutainers can be used for sample collection, as can short butterfly catheters. For very small or pediatric patients, samples can be placed into microtainer tubes. Regardless of the tube used for sample collection, the blood must be adequately mixed with the anticoagulant during and after collection to prevent clotting. This is done by gentle inversion of the tube (tubes should never be shaken).