Question

write an activity for the test for presence of glucose buy bi bendix experiment​

Answer 1

Answer : the activity is as follows:

Aim

To carry out appropriate tests to detect the presence of sugar(sucrose) in suitable animal and plant materials.

The presence of sucrose can be tested in a sample using Benedict’s test.

Material Required

• Benedict’s Reagent

• Sugarcane extract
• Concentrated HCl
• NaOH solution
• Burner
• Dropper
• Test tube holder

Procedure

• Add a few drops of concentrated HCl using a dropper to the test tube that contains sugarcane extract

• Bring the contents of the test tube to a boil for 2 minutes.

• Hold the test tube securely with the help of a test tube holder

• Hydrolysis of sucrose takes place into fructose and glucose

• With the help of a dropper, add a few drops of NaOH solution to the test tube so as the solution turns alkaline

Perform Benedict’s test to the solution to detect the presence of glucose.

• Add a few drops of Benedict’s reagent with the help of a dropper into the test tube holding the sample

• Hold the test tube securely with the help of a test tube holder, bring the sample to a boil over a burner for a few minutes.

Observation

The color of the solution switches from blur color to green color. From green, the color is finally switched to brick red or orange. This indicates that the solution contains glucose.

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