Question

write an activity to observe fungal hyphae under microscope​

Answer 1

Answer:

The soil sample from the rhizoshere of the roots of plants will be collected at different stages. 100gm of the fine soil will be take and remove the debris and other larger particles and dissolved in the water and kept for about 24 hours’ duration when the soil will completely settled down in the bottom of the beaker the water will passed through 2mm sieve to remove the remaining residues. The cleaned water then passed from the three different sizes of sieves i.e. 140, 170 and 400 µm one by one. The remaining residues above the sieves will be collected by rubbing the filter paper on the sieves and studied under the compound microscope for the fungal species diversity and density. The spores will be collected through needle and kept on the drop of candabalsam and the slides will be made then the picture will be capture for identification. For the identifications the manual of Hall and Fish (1979), Trappe, (1982) and Schenck and Perez (1990) were adopted. The spore’s density was calculated.

For the root infection observations, the standard protocol of Giovannetti and Mosse (1980) and Kormanik (1982) will be followed. The roots of the plants were washed with tap water to remove the formaline acetic acid molecules and then cut into pragments and heated for 10-15 minutes in 10% KOH solution. For the bleaching of the pigmented portions of the roots were then kept in alkalineH2O2 for about 10-15 minutes. The bleached roots segments were then washed with tape water to remove the H2O2. After that the segments were treated with 1% HCL for about 1-2 minutes to keep the acidic effect for proper staining. In 0.025% acidic fuchsine the roots segments will be kept and heated for 2-3 minutes. Ten segments of about 1cm length will be randomly collected and kept under microscope for the morphological studies of AMF entophyte and microphotographs will be taken. The percentage of infections will be calculated .

Explanation:

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Answer 2

Explanation:

• Fungi have mycelium an spores which are used in identification

• Place a small piece of fungus containing the structures that you wish to see onto a slide and mount in a drop of water.

• Place a cover slip on top.

• Gently tap the specimen.

• Check under a compound microscope.