write an activity to show the tissue culture at home with diagram
Answers
Answer:
1 vial of Murashige Skoog (MS) medium. (If you wish to make up your own growing medium you could use the recipe for the Murashige medium given at the end of this section.)
1 L sterile distilled water
8 g of agar/L
30 g sucrose/L
1.5-L or 2-L container in which to prepare the growth medium
Small amounts of 1M NaOH and 1M HCl to adjust the pH of the medium
30 flat-bottom culture tubes with closures
Glass aquarium or box lined with plastic
Plastic sheet to cover the top of the aquarium
Adhesive tape
10% bleach in a spray bottle
70% alcohol in a spray bottle
Forceps or tweezers
Gloves
Cutting equipment such as a scalpel blade or razor blade
2 bottles of sterile distilled water (purchase at the grocery store)
Pressure cooker
Your chosen plant (cauliflower, rose, African violet, or carnation)
Paper towel for cutting on, or sterile petri dishes if available
Beaker or container in which to wash the plant material
Detergent-water mixture - 1 ml detergent per liter of water
Bleach sterilizing solution - dilute commercial bleach (5-6% sodium hypochlorite) to a final concentration of 1-2% sodium hypochlorite in distilled water in a large beaker or jar.
2 or 3 beakers or containers of sterile water
A well-lit area away from direct sunlight or use full-spectrum gro-lights
Hormones such as BAP (benzylaminopurine) and NAA (naphthalene acetic acid) to stimulate growth and root development, respectively. (Commercial rooting hormone solutions and powders are also available from hardware stores.)