Question

write correct sequence of four steps for the preparation of temporary mount of a stained leaf peel

Answer 1

correct sequence is
1. Pull out a thin peel from the lower surface of leaf.
2. Stain the peel in safranine.
3. Remove the extra stain by washing with water.
4. Place a drop of glycerine on the slide.
5. Place the stained peel on the glycerine.
6. Place the cover slip over the peel.

Answer 2

Explanation:

i) Take a healthy leaf from the potted plant.

(ü) Remove a part of the peel from the lower surface of the leaf. You can do this

by folding the leaf over and gently pulling the peel apart using forceps. Keeps

the peel in a watch glass containing water.

(iii) Put a few drops of safranin stain in a watch glass.

v) After 2-3 minutes take out the peel and place it on a clean glass slide.

() Put a drop of glycerin over the peel and place a clean coverslip gently over it

with the help of a needle.

(vi)

Ans.

Remove the excess stain and glycerin with the help of blotting paper.

(vi) Observe the slide under magnifications of the compound microscope.