Question

Write correct sequence of four steps of method for the preparation of temporary mount of a obtained leaf peel to show its stomata

Answer 1

Real Lab Procedure

Pluck a fresh leaf from a balsam plant.Fold the leaf and carefully tear along the bruised area of the lower side of the leaf.We can see a colourless narrow border along the torn edge.Carefully pull out the thin membranous transparent layer from the lower epidermis using a forceps.Put the epidermis into a watch glass containing distilled water.Take few drops of Safranin solution using a dropper and transfer this into another watch glass.Using a brush transfer the epidermis into the watch glass containing the Safranin solution.Keep the epidermis for 30 sec in the Safranin solution to stain the peel.To remove excess stain sticking to the peel, place it again in the watch glass containing water.Place the peel onto a clean glass slide using the brush.Take a few drops of glycerine using a dropper and pour this on the peel.Using a needle, place a cover slip over the epidermis gently.Drain out the excess glycerine using a blotting paper.Take the glass slide and place it on the stage of the compound microscope.Examine the slide through the lens of the compound microscope.

Hope this helps you.

Answer 2

wash the leaf with alcohol
remove the peel gently
put the thinnest layer on to the slide and add stain(safranine)and glycerine and remove the access stain using tissue paper
put the cover slip such that no any air bubble there under the cover slip
Slide is ready dear
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