Question

write correct sequence of four steps of methods for the preparation of temporary mount of a stained leaf peel

Answer 1

The correct sequence is
1. Pull out a thin peel from the lower surface of leaf.
2. Stain the peel in safranine.
3. Remove the extra stain by washing with water.
4. Place a drop of glycerine on the slide.
5. Place the stained peel on the glycerine.
6. Place the cover slip over the peel.

Answer 2

Answer: The following steps are necessary to provide a temporary mount for the stained leaf are:

Explanation:

• A healthy leaf is taken and peel is removed from its lower surface.  Keep the peel in a watch glass with water in it.

• You should put a few drops of safranin stain in that watch glass. After two to three minutes, remove the peel and set it on a clean glass slide.
• A drop of glycerin is applied to the peel and gently cover it with a clean coverslip and remove any extra glycerin or stains with blotting paper.

• Use the compound microscope to examine the slide at various magnifications.

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