Question

write down different surface sterilizing agent commonly used in laboratory

Answer 1

Hey..mate here is yur ans...
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1) $\textbf{DRY HEAT(FLAMING)}$
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Dry heating is generally different from Autocalving Dry heat kill microbes by oxidation of cellular components. This requires more energy than protein hydrolysis so higher temperatures are required for efficient sterilization by dry heat.
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For example sterilisation can normally be achieved in 15 minutes by autoclaving at 121° C where as dry heating would generally need a temperature of 160° C to sterilize in a similar amount of time.
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2)$\textbf{WET HEAT(AUTOCALVING)}$
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AUTOCALVING is one of the mist important method to be sterilizatiousing Auto calving kill endospores. This is a very effective method that kills all microbes, spores and viruses, although for some specific bugs, especially high temperatures or Short time are required.
Autoclaving kills microbes by hydrolysis and coagulation(clotting) of cellular proteins, which is efficiently achieved by intense heat in the presence of water.The intense heat comes from the steam.
IT is the best method of STERILIZATION
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3)$\textbf{RADIATION}$
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In this method UV, x-rays and gamma rays are all types of electromagnetic radiation that have profoundly damaging effects on DNA are required.UV has limited penetration in air so sterilisation only occurs in a small area around the lamp. While, it is relatively safe and is quite useful for sterilising small areas, like laminar flow hoods.
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