write down tha detail note on method of isotonicity
Answers
Cryoscopic method
In this method, the quantity of each substance required for an isotonic solution can be calculated from the freezing point depression values. A solution which is isotonic with blood has a of 0.52ᵒC. Therefore, the freezing point of drug solution must be adjusted to this valve.
Isotonicity valve is calculated by using the hemolytic method in which the effect of various solutions of drug is observed on the appearance of red blood cells suspended in solution.
Answer:
The three frequently used methods to calculate isotonicity of the solutions are described below. Class-1 Methods: Nacl or some other substances is added to the solution of the drug to lower the freezing point of the solution to -0.52ᵒC and thus make the solution isotonic