write down the process of test of protein
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To a test tube, add 40 drops of liquid to be tested.
If testing more than one liquid, label each test tube with a marker.
Add 3 drops of Biuret reagent solution to each test tube. Shake gently to mix.
Note any color change. Proteins will turn solution pink or purple.
Biuret Test
Biuret Test: The Biuret Test is a general test for proteins. When a protein reacts with copper(II) sulfate (blue), the positive test is the formation of a violet colored complex. The Biuret Test works for any compound containing two or more of the following groups.
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- ᴛᴏ ᴀ ᴛᴇsᴛ ᴛᴜʙᴇ, ᴀᴅᴅ ᴅʀᴏᴘs ᴏғ ʟɪǫᴜɪᴅ ᴛᴏ ʙᴇ ᴛᴇsᴛᴇᴅ.
- ɪғ ᴛᴇsᴛɪɴɢ ᴍᴏʀᴇ ᴛʜᴀɴ ᴏɴᴇ ʟɪǫᴜɪᴅ, ʟᴀʙᴇʟ ᴇᴀᴄʜ ᴛᴇsᴛ ᴛᴜʙᴇ ᴡɪᴛʜ ᴀ ᴍᴀʀᴋᴇʀ.
- ᴀᴅᴅ ᴅʀᴏᴘs ᴏғ ʙɪᴜʀᴇᴛ ʀᴇᴀɢᴇɴᴛ sᴏʟᴜᴛɪᴏɴ ᴛᴏ ᴇᴀᴄʜ ᴛᴇsᴛ ᴛᴜʙᴇ. sʜᴀᴋᴇ ɢᴇɴᴛʟʏ ᴛᴏ ᴍɪx.
- ɴᴏᴛᴇ ᴀɴʏ ᴄᴏʟᴏʀ ᴄʜᴀɴɢᴇ. ᴘʀᴏᴛᴇɪɴs ᴡɪʟʟ ᴛᴜʀɴ sᴏʟᴜᴛɪᴏɴ ᴘɪɴᴋ ᴏʀ ᴘᴜʀᴘʟᴇ.
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