Question

write down the process (or steps)invloued in tissue culture

Answer 1

Tissue culture is a process that involves exposing plant tissue to a specific regimen of nutrients, hormones, and light under sterile, in vitro conditions to produce many new plants, each a clone of the original mother plant, over a very short period of time. ... There are three main steps to the tissue culture process

The Tissue Culture Process

Stage 1 Initiation. We take a piece of plant tissue (bud, leaf, stem or root) and disinfect it as much as possible using sterile water, bleach and alcohol. ...

Stage 2 Multiplication. ...

Stage 4 Pre-Rooting.

Answer 2

Main steps in Tissue Culture are:-

1. Selection of plant
2. Isolation of explant
3. Sterilization of explant
4. Inoculation of explant
5. Incubation
6. Initiation of callus
7. Sub-culturing
8. Regeneration
9. Hardening