Write down the steps you will follow to prepare temporal slides of
1) plant epidermis eg.onion,talinium,rheo.
2) Human check cell .
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To Prepare Stained Temporary Mount of Onion Peel
Materials Required
Real Lab Procedure
Pour some distilled water into a watch glass.
Peel off a leaf from half a piece of onion and using the forceps, pull out a piece of transparent onion peel (epidermis) from the leaf.
Put the epidermis in the watch glass containing distilled water.
Take a few drops of safranin solution in a dropper and transfer this into another watch glass.
Using a brush, transfer the peel into the watch glass containing the safranin solution.
Let this remain in the Safranin solution for 30 seconds, so that the peel is stained.
Take the peel from the Safranin solution using the brush and place it in the watch glass containing the distilled water.
Take a few drops of glycerine in a dropper and pour 2-3 drops at the center of a dry glass slide.
Using the brush, place the peel onto the slide containing glycerine.
Take a cover slip and place it gently on the peel with the aid of a needle.
Remove the extra glycerine using a piece of blotting paper.
Place this glass side on the stage of the compound microscope and view it.
Observations
There are a large number of regularly shaped cells lying side by side and each cell has a distinct cell wall.
A distinct nucleus is present on the periphery of each cell.
Lightly stained cytoplasm is observed in each cell.
A large vacuole is present at the centre of each cell, and is surrounded by the cytoplasm.
Conclusion
As cell walls and large vacuoles are clearly observed in all the cells, the cells placed for observation are plant cells.
Precautions
Use a brush to transfer the peel from one apparatus to another.
Staining of peel should neither be too dark, nor too light.
Extra glycerine stain should be removed using blotting paper.
To Prepare Stained Temporary Mount of Human Cheek Cells
Materials Required
Real Lab Procedure
Gently scrape the inner side of the cheek using a toothpick, which will collect some cheek cells.
Place the cells on a glass slide that has water on it.
Mix the water and the cheek cells using a needle and spread them.
Take a few drops of Methylene blue solution using a dropper and add this to the mixture on the slide.
After 2-3 minutes remove any excess water and stain from the slide using a blotting paper.
Take a few drops of glycerine using a dropper and add this to the test mixture.
Take a clean cover slip and lower it carefully on the mixture with the aid of a needle.
Using a brush and needle, press the cover slip gently to spread the epithelial cells.
Remove any extra liquid around the cover slip using a blotting paper.
Place this glass side on the stage of the compound microscope and view it.
Observations
A large number of flat and irregular-shaped cells are observed.
The cells do not have a cell wall. However, each cell has a thin cell membrane.
A deeply stained nucleus is observed at the centre of each cell.
No prominent vacuoles are observed in the cells.
Conclusion
As the cells observed do not have a cell wall, nor a prominent vacuole, the cells of the specimen on the slide are animal cells.
Materials Required
Real Lab Procedure
Pour some distilled water into a watch glass.
Peel off a leaf from half a piece of onion and using the forceps, pull out a piece of transparent onion peel (epidermis) from the leaf.
Put the epidermis in the watch glass containing distilled water.
Take a few drops of safranin solution in a dropper and transfer this into another watch glass.
Using a brush, transfer the peel into the watch glass containing the safranin solution.
Let this remain in the Safranin solution for 30 seconds, so that the peel is stained.
Take the peel from the Safranin solution using the brush and place it in the watch glass containing the distilled water.
Take a few drops of glycerine in a dropper and pour 2-3 drops at the center of a dry glass slide.
Using the brush, place the peel onto the slide containing glycerine.
Take a cover slip and place it gently on the peel with the aid of a needle.
Remove the extra glycerine using a piece of blotting paper.
Place this glass side on the stage of the compound microscope and view it.
Observations
There are a large number of regularly shaped cells lying side by side and each cell has a distinct cell wall.
A distinct nucleus is present on the periphery of each cell.
Lightly stained cytoplasm is observed in each cell.
A large vacuole is present at the centre of each cell, and is surrounded by the cytoplasm.
Conclusion
As cell walls and large vacuoles are clearly observed in all the cells, the cells placed for observation are plant cells.
Precautions
Use a brush to transfer the peel from one apparatus to another.
Staining of peel should neither be too dark, nor too light.
Extra glycerine stain should be removed using blotting paper.
To Prepare Stained Temporary Mount of Human Cheek Cells
Materials Required
Real Lab Procedure
Gently scrape the inner side of the cheek using a toothpick, which will collect some cheek cells.
Place the cells on a glass slide that has water on it.
Mix the water and the cheek cells using a needle and spread them.
Take a few drops of Methylene blue solution using a dropper and add this to the mixture on the slide.
After 2-3 minutes remove any excess water and stain from the slide using a blotting paper.
Take a few drops of glycerine using a dropper and add this to the test mixture.
Take a clean cover slip and lower it carefully on the mixture with the aid of a needle.
Using a brush and needle, press the cover slip gently to spread the epithelial cells.
Remove any extra liquid around the cover slip using a blotting paper.
Place this glass side on the stage of the compound microscope and view it.
Observations
A large number of flat and irregular-shaped cells are observed.
The cells do not have a cell wall. However, each cell has a thin cell membrane.
A deeply stained nucleus is observed at the centre of each cell.
No prominent vacuoles are observed in the cells.
Conclusion
As the cells observed do not have a cell wall, nor a prominent vacuole, the cells of the specimen on the slide are animal cells.
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