Question

write four steps in proper sequence in the preparation of temporary Mount of a stained leaf peel

Answer 1

1. Pull out a thin peel from the lower surface of leaf.
2. Stain the peel in safranine.
3. Remove the extra stain by washing with water.
4. Place a drop of glycerine on the slide.
5. Place the stained peel on the glycerine.
6. Place the cover slip over the peel.

Answer 2

The four proper sequence of the preparation of temporary mount of a stained leaf are as follows:

• A leaf is taken and folded in the centre of the leaf to break it in two pieces.

• A leaf piece is pulled to get a transparent leaf peel.

• The lower epidermis leaf peel is cut into small pieces and is put into a dish with water.

• A selected leaf peel is transferred into a dilute solution of safrain.