Biology, asked by naniashahanu7698, 1 year ago

Write in detail any two vectors used in gene cloning.

Answers

Answered by ajit12345

In the molicular cloning workflow, the DNA to be cloned is identified and treated with enzymes to generate DNA fragments. These fragments are then combined with vector DNA to generate recombinant DNA molicule. Bacterial expression and ligase-independent cloning kits,enzymes,and DNA vectors you need for efficient molecular cloning.

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Answered by sailorking

The two vectors used in gene cloning are the plasmids and phages.

The size of a plasmid are generally 4361 bp
The cloning limit of a plasmid is 0.1-10kb.
ampicillin and the tetracycline resistant gene are the marker genes which remains isolated from the E-coli.

The size of a bacteriophage is 48502 bp but one third of this part is not at all essential
These are the ones those who infects bacteria.
It is formed by nucleic acid molecule.

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