write procedse of observing bactess
Answers
Answer:
Most people do not have a phase contrast microscope, which makes it much easier to see bacteria. It is still possible to see them, if your microscope has a condenser. Here are some suggestions.
Use the 40x objective: With the 10x eyepiece you get 400x total magnification. This is enough to see them. Of course you have to start focusing with the 4x and 10x objective first and then work your way upwards.
Completely close the condenser: This will increase contrast and depth of field. If your microscope does not have a condenser, then you might not be able to see them. In this case I suggest that you buy some ready-made slides with stained bacteria.
Use very little water: When making the slide, use very little water and make sure that there are no larger particles under the cover glass. There must be a thin film of water beneath the cover glass. Otherwise the bacteria can float vertically and go out of focus. The cover glass should not float on the water. As a matter of fact, it is better if the water is so little that it is not even able to spread beneath the whole cover glass.
Keep bacterial density low: If there are too many bacteria in the sample, then they will overlap and you will only see a clump.