Question

Write short notes on Denaturation of proteins

Answer 1

Denaturation: Disruption of protein Secondary, Tertiary, and Quaternary structure.  

Protein Tertiary and Quaternary structures are made up of weak Hydrogen and ionic bonds. Due to denaturing factors, these bonds break freeing up the primary or secondary structure. But Primary structure is strong and denaturing factors/reactions are not strong enough to break the primary structure, this structure remains intact after the process. 

Factors/reactions that cause the denaturation are
- Heat
- Alcohol: Ethanol
- Treatment with Acid or base: Acetic Acid, Sulfosalicyclic acid
- Heavy Metal salts: Dithiothreitol
- Chaotropic agents: Urea


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Answer 2

Denaturation:

The process of a protein - losing its higher order structure without losing the primary structure, it called denaturation.