Write short notes on Digestion methods in mass spectroscopy.
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The most widely applied method still is bottom-up proteomics where the protein is digested into peptides that can be efficiently analyzed with a wide range of LC–MS or MALDI-TOF-MS instruments. ... The most crucial step in such approaches is the protein digestion, which is often the bottleneck in terms of time consumption.
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Mass spectrometry (MS) is an analytical technique that ionizes chemical species and sorts the ions based on their mass-to-charge ratio. In simpler terms, a mass spectrummeasures the masses within a sample. Mass spectrometry is used in many different fields and is applied to pure samples as well as complex mixtures.
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