Question

write steps involed in micropropogation in plants​

Answer 1

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The different stages of micropropagation include:

1. Selection of mother plant.
2. Establishment of aseptic culture.
3. Shoot multiplication.
4. Root multiplication.
5. Transplantation.

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Answer 2

Answer:

Stages of Micropropagation

Stages

Methods Involved

Stage 0

Selection of an explant

Stage I

Culture initiation and establishment

Stage II

Shoot multiplication

Stage III

Rooting of the shoots

Stage IV

Transfer of plantlets in the greenhouse environment

Stage 0

This is the initial stage of micropropagation. The stock plants are selected and grown under controlled conditions before using them for culture initiation.

Stage I

The explants are established in a suitable culture medium. This stage involves the following steps:

Isolation of the explant

Surface Sterilization

Washing

The explant is established on an appropriate culture medium.

Stage II

This stage involves the rapid multiplication of shoots or rapid somatic embryo formation in a defined culture medium.

Stage III

In this stage, the shoots are transferred to a medium for the development of roots. The shoots are either transferred directly in the soil for the root development or transferred to a nutrient medium in the laboratory.

Stage IV

In this stage, the plantlets are established in the soil. The shoots from the laboratory are transferred to a greenhouse under controlled conditions of temperature, humidity and light.