Write Ten difference between LSC and LLC ?
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Liquid–liquid chromatography (LLC) is liquid partition chromatography in which the solid phase serves as a support for a stationary liquid phase.
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Liquid – Solid Chromatography (LSC)
The solid stationary phase serves as a selective adsorbent for the sample components. The mode of separation can be either two- dimensional as in thin layer chromatography or three-dimensional as on the stationary phase packed inside the chromatographic column.
Physical forces, typically, van der waal’s interactions are responsible for temporary retention of sample components. Due to the degree of interaction there is a steady equilibrium which replaces the retained molecules from others present in the mobile phase resulting in separation and selective extrusion of the adsorbed molecules. Separation factor α is a quantitative measure of degree of separation under a given set of conditions. It is the ratio of two components in the adsorbing phase divided by their ratio in the mobile phase.
Liquid – Liquid Chromatography (LLC)
The basics of liquid-liquid separation is to partition solutes between two immiscible liquid phases- one phase being the mobile carrier and the other being the thin liquid layer supported on the inert stationary phase packed inside the column. It is analogous to separation between two immiscible liquids with the help of a conventional separating funnel. The concurrent extraction process as the mobile phase travels down the column is much more efficient and faster than a single stage extraction using the separating funnel .It also results in clearly defined isolation bands which can be easily detected using a suitable detector.
The solid stationary phase serves as a selective adsorbent for the sample components. The mode of separation can be either two- dimensional as in thin layer chromatography or three-dimensional as on the stationary phase packed inside the chromatographic column.
Physical forces, typically, van der waal’s interactions are responsible for temporary retention of sample components. Due to the degree of interaction there is a steady equilibrium which replaces the retained molecules from others present in the mobile phase resulting in separation and selective extrusion of the adsorbed molecules. Separation factor α is a quantitative measure of degree of separation under a given set of conditions. It is the ratio of two components in the adsorbing phase divided by their ratio in the mobile phase.
Liquid – Liquid Chromatography (LLC)
The basics of liquid-liquid separation is to partition solutes between two immiscible liquid phases- one phase being the mobile carrier and the other being the thin liquid layer supported on the inert stationary phase packed inside the column. It is analogous to separation between two immiscible liquids with the help of a conventional separating funnel. The concurrent extraction process as the mobile phase travels down the column is much more efficient and faster than a single stage extraction using the separating funnel .It also results in clearly defined isolation bands which can be easily detected using a suitable detector.
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