Write the apparatus and procedure, followed by you in the laboratory to observe the Nucleus in the cell ?
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Answers
Answer:
To Prepare Stained Temporary Mount of Human Cheek Cells :-
Materials Required:
Real Lab Procedure Gently scrape the inner side of the cheek using a toothpick, which will collect some cheek cells.Place the cells on a glass slide that has water on it.Mix the water and the cheek cells using a needle and spread them.Take a few drops of Methylene blue solution using a dropper and add this to the mixture on the slide.After 2-3 minutes remove any excess water and stain from the slide using a blotting paper.Take a few drops of glycerine using a dropper and add this to the test mixture.Take a clean cover slip and lower it carefully on the mixture with the aid of a needle.Using a brush and needle, press the cover slip gently to spread the epithelial cells.Remove any extra liquid around the cover slip using a blotting paper.Place this glass side on the stage of the compound microscope and view it.
Observations:
A large number of flat and irregular-shaped cells are observed.The cells do not have a cell wall. However, each cell has a thin cell membrane.A deeply stained nucleus is observed at the centre of each cell.No prominent vacuoles are observed in the cells.
Conclusion:
As the cells observed do not have a cell wall, nor a prominent vacuole, the cells of the specimen on the slide are animal cells.