Question

Write the application of cytokinins in tissue culture​

Answer 1

Cytokinins (CK) are a class of plant growth substances (phytohormones) that promote cell division, or cytokinesis, in plant roots and shoots. They are involved primarily in cell growth and differentiation, but also affect apical dominance, axillary bud growth, and leaf senescence. Folke Skoog discovered their effects using coconut milk in the 1940s at the University.

There are two types of cytokinins: adenine-type cytokinins represented by kinetin, zeatin, and 6-benzylaminopurine, and phenylurea-type cytokinins like diphenylurea and thidiazuron (TDZ).[2] Most adenine-type cytokinins are synthesized in roots.[3] Cambium and other actively dividing tissues also synthesize cytokinins.[4] No phenylurea cytokinins have been found in plants.

Answer 2

In biological research, tissue culture refers to a method in which fragments of a plant or animal tissue are introduced into a new, artificial environment, where they continue to function or grow.

While fragments of a tissue are often used, it is important to note that entire organs are also used for tissue culture purposes. Here, such growth media as broth and agar are used to facilitate the process.

* While the term tissue culture may be used for both plant and animal tissues, plant tissue culture is the more specific term used for the culture of plant tissues in tissue culture.