Question

write the features of cloning vector

Answer 1

Characteristics of a cloning vectors

• It must be small in size.
• It must be self-replicating inside host cell.
• It must possess restriction site for Restriction Endonuclease enzymes.
• Introduction of donor DNA fragment must not interfere with replication property of the vector.

Answer 2

Answer:

Characteristics of a cloning vectors

it must be small in size

It must be self-replicating inside host cell

It must possess restriction site for Restriction Endonuclease enzymes

Introduction of donor DNA fragment must not interfere with replication property of the vector

It must possess some marker gene such that it can be used for later identification of recombinant cell

it must possess multiple cloning site