write the name of difference types of chromography
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There are four main types of chromatography. These are Liquid Chromatography, Gas Chromatography, Thin-Layer Chromatography and Paper Chromatography...
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Types of Chromatography
The four main types of chromatography are
1. Adsorption Chromatography
In the process of adsorption chromatography, different compounds are adsorbed on the adsorbent to different degrees based on the absorptivity of the component. Here also, a mobile phase is made to move over a stationary phase, thus carrying the components with higher absorptivity to a lower distance than that with lower absorptivity.
2. Thin Layer Chromatography
In the process of thin-layer chromatography (TLC), the mixture of substances is separated into its components with the help of a glass plate coated with a very thin layer of adsorbent, such as silica gel and alumina.
The plate used for this process is known as chrome plate. The solution of the mixture to be separated is applied as a small spot at a distance of 2 cm above one end of the plate. The plate is then placed in a closed jar containing a fluid termed as an eluant, which then rises up the plate carrying different components of the mixture to different heights.
3. Column Chromatography
Column chromatography is the technique used to separate the components of a mixture using a column of suitable adsorbent packed in a glass tube, as shown in the figure below. The mixture is placed on the top of the column, and an appropriate eluant is made to flow down the column slowly.
Depending upon the degree of adsorption of the components on the wall adsorbent column, the separation of the components takes place. The component with the highest absorptivity is retained at the top, while the other flow down to different heights accordingly.
4. Partition chromatography
In this process, a continuous differential partitioning of components of a mixture into a stationary phase and mobile phase takes place. The example of partition chromatography can be seen in paper chromatography. In this process, chromatography paper is used as a stationary phase which is suspended in a mixture of solvents that act as a mobile phase.
Here, we put a spot at the base of the chromatographic paper with the mixture to be separated and as the solvent rises up this paper, the components are carried to different degrees depending upon their retention on the paper. The components are thus separated at different heights.
Hope it’s clearly explained about your question.
The four main types of chromatography are
1. Adsorption Chromatography
In the process of adsorption chromatography, different compounds are adsorbed on the adsorbent to different degrees based on the absorptivity of the component. Here also, a mobile phase is made to move over a stationary phase, thus carrying the components with higher absorptivity to a lower distance than that with lower absorptivity.
2. Thin Layer Chromatography
In the process of thin-layer chromatography (TLC), the mixture of substances is separated into its components with the help of a glass plate coated with a very thin layer of adsorbent, such as silica gel and alumina.
The plate used for this process is known as chrome plate. The solution of the mixture to be separated is applied as a small spot at a distance of 2 cm above one end of the plate. The plate is then placed in a closed jar containing a fluid termed as an eluant, which then rises up the plate carrying different components of the mixture to different heights.
3. Column Chromatography
Column chromatography is the technique used to separate the components of a mixture using a column of suitable adsorbent packed in a glass tube, as shown in the figure below. The mixture is placed on the top of the column, and an appropriate eluant is made to flow down the column slowly.
Depending upon the degree of adsorption of the components on the wall adsorbent column, the separation of the components takes place. The component with the highest absorptivity is retained at the top, while the other flow down to different heights accordingly.
4. Partition chromatography
In this process, a continuous differential partitioning of components of a mixture into a stationary phase and mobile phase takes place. The example of partition chromatography can be seen in paper chromatography. In this process, chromatography paper is used as a stationary phase which is suspended in a mixture of solvents that act as a mobile phase.
Here, we put a spot at the base of the chromatographic paper with the mixture to be separated and as the solvent rises up this paper, the components are carried to different degrees depending upon their retention on the paper. The components are thus separated at different heights.
Hope it’s clearly explained about your question.
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