Write the principle and applications of the following processes;
1. Chromatography 2. Centrifugation
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chromatography summary
This chapter discusses planar chromatography and column chromatography. The objective of chromatography is to separate the various substances that make up a mixture. The applications range from a simple verification of the purity of a given compound to the quantitative determination of the components of a mixture. The chromatographic system consists of a fixed phase and a moving phase. The mixture to be analyzed or solute is introduced into the system through the mobile phase, and it is the affinity of the solute for one phase over the other which governs its separation from the other components. Each component is retained to a different degree in the system and retention is based on various attraction forces. A chromatogram is a graphical representation of the compounds eluting from a chromatographic system. Chromatography can be used for qualitative and quantitative determination of various food constituents.
This chapter discusses planar chromatography and column chromatography. The objective of chromatography is to separate the various substances that make up a mixture. The applications range from a simple verification of the purity of a given compound to the quantitative determination of the components of a mixture. The chromatographic system consists of a fixed phase and a moving phase. The mixture to be analyzed or solute is introduced into the system through the mobile phase, and it is the affinity of the solute for one phase over the other which governs its separation from the other components. Each component is retained to a different degree in the system and retention is based on various attraction forces. A chromatogram is a graphical representation of the compounds eluting from a chromatographic system. Chromatography can be used for qualitative and quantitative determination of various food constituents.
yopoke408:
I cant understand what is the principle and what is the application
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chromatography
principle: the samples are subjected to flow by mobile liquid onto or through a stable stationary phase.
application: testing water samples, also checks air quality
Centrifugation
principle: the high rate of acceleration cause the dense substance to settle down letting the lighter one go upwards
application: getting butter from cream etc.
principle: the samples are subjected to flow by mobile liquid onto or through a stable stationary phase.
application: testing water samples, also checks air quality
Centrifugation
principle: the high rate of acceleration cause the dense substance to settle down letting the lighter one go upwards
application: getting butter from cream etc.
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