write the principle involved in assay of calcium gluconate
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Principle involved in assay of calcium gluconate:
Explanation:
Principle involved in assay of calcium gluconate are:
- The assay of calcium gluconate relies upon a replacement complexometric titration. Magnesium forms a fancy with the mixture of indicator mordant black II which indicates the primary color.
- The magnesium-indicator complex is more stable compared to the calcium-indicator complex; hence calcium doesn't affect the magnesium-indicator complex. When titrated against disodium edetate, calcium and EDTA formed.
- When all of the calcium has been consumed, the drop of EDTA breaks the magnesium-indicator complex, allowing the free indicator to make a fancy with the magnesium. The endpoint is decided by detecting the second color at that point.
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