Question

Write the principle of Southern blotting.

Answer 1

Explanation:

Southern blotting is a hybridization technique for identification of particular size of DNA from the mixture of other similar molecules. This technique is based on the principle of separation of DNA fragments by gel electrophoresis and identified by labelled probe hybridization.

Answer 2

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Southern blotting is a hybridization technique for identification of particular size of DNA from the mixture of other similar molecules. This technique is based on the principle of separation of DNA fragments by gel electrophoresis and identified by labelled probe hybridization.

Step-by-Step Guide to Southern Blot Analysis

• Step 1 DNA digestion.

• Step 2 Gel electrophoresis.

• Step 3 Blotting.

• Step 4 Probe labeling.

• Step 5 Hybridization & washing.

• Step 6 Detection.

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