Question

write the principle of staining ​

Answer 1

Answer:

Principle. Its principle is based on the principle of producing a marked contrast between the organism and around its surrounding, by the use of basic stain. A basic dye consists of positive chromophore which strongly attracts to the negative cell components and charged molecules like nucleic acids and proteins.

Answer 2

Staining is a technique used to enhance contrast in samples, generally at the microscopic level. Stains and dyes are frequently used in histology (the study of tissue under the microscope) and in the medical fields of histopathology, hematology, and cytopathology that focus on the study and diagnoses disease at a microscopic level. Stains may be used to define biological tissues (highlighting, for example, muscle fibers or connective tissue), cell populations (classifying different blood cells), or organelles within individual cells.

In biochemistry it involves adding a class-specific (DNA, proteins, lipids, carbohydrates) dye to a substrate to qualify or quantify the presence of a specific compound. Staining and fluorescent tagging can serve similar purposes. Biological staining is also used to mark cells in flow cytometry, and to flag proteins or nucleic acids in gel electrophoresis.

Staining is not limited to biological materials, it can also be used to study the structure of other materials for example the lamellar structures of semi-crystalline polymers or the domain structures of block copolymers.


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