Question

write the procedure of onion peel experiment

Answer 1

$\bold{\large{\underline{Answer\: \colon}}}$


$\bold{\large{\underline{Aim\: \colon}}}$


To mount the $\bold{onion\: peel}$ on the slide.


$\bold{\large{\underline{Material\: required\: \colon}}}$


$\bold{Safranin}$ , cotton , onion , cloth & glycerin.


$\bold{\large{\underline{Instrument \: and \: laboratory \: tools}}}$


$\bold{Light\:Microscope}$

Slide , forcepe , glass , coverslip , knife , needle.

$\bold{\large{\underline{Procedure\: \colon}}}$


1] Cut the onion and remove it's peel from the inner surface of onion.

2] Put the peel in a $\bold{watch\: glass}$ containing water.

3] Add few drops of $\bold{safranin\: stain}$ into the $\bold{watch\: glass}$ to stain the peel.

4] Remove the peel with the help of $\bold{ camel\: hair \: brush}$ and put it on clean $\bold{slide}$ along with few drops of water and provide the shape by cutting it.

5] Wash $\bold{extra\: strain}$ with water and remove $\bold{excess\: water}$ with the help of $\bold{filter \: paper}$.

6] Put the drop of $\bold{glycerine}$ on the slide over the peel as required and clean the excess with filter paper.

7] Now put $\bold{coverslip}$ over the peel with help of $\bold{needle}$.

8] The temporary mount of onion peel is ready for abservation in under the $\bold{microscope}$.

$\bold{\large{Precautions\: \colon}}$

1] Slide is clean and dry .

2] Peel should be mounted in the $\bold{ center}$ of slide.

3] Use always brush to transfer peel from watch glass to slide.

4] Avoid the entry of air bubbles in inside during mounting.

$\bold{\large{Note \: \colon}}$


Wash hands during experiment and under the $\bold{supervision}$.


$\bold{\large{\underline{Thanks}}}$

Answer 2

separated out a thin onion scale from onion.

tear it from the concave side so as get a transparent , thin and membranous onion peel called epidermis.

now keep this onion peel piece in a watch glass containing water.

cut out a small portion of this peel and place it flat on aglass slide on a drop of water with the help of a thin camel hair paint brush .

add a drop of iodine solution or methylene blue stain .


drain out the excess stain and mount the onion peel in a drop of glycerine under a coverslip .

examine the slide under low and high power of a compound microscope.