Question

Write the procedure to observe lactobacillus in buttermilk

Answer 1

Take few drops of buttermilk and dilute it with few drops of water.
Put a drop of crystal violet and cover with a cover slip and allow it to get stained fro 1 min.
Wash the slide to remove excess of stain and observe it under microscope.
The dark blue coloured rod shaped microscope are the Lactobacillus.
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Answer 2

$\textbf{ANSWER}$



TO OBSERVE THE LACTOBACILLI IN BUTTERMILK :

• PROCEDURE :

1. Take a very small drop of curd on the glass slide. With the help of a toothpick smear it continuously till a thin film of the curd is formed on the slide.

2. Let the smear dry.

3. Place a small drop of methylene blue solution on a microscope slide. This is staining the slide.

4. Place a coverslip on top. Remove excess solution around the coverslip with a blotting paper.

5. Place the slide on the stage of the microscope starting with lower magnification. After observing the bacteria under low power as tiny dots, shift the eyepiece to the high-power magnification.