write the procedure to test proteins
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Answer:
What to do.
- To a test tube, add 40 drops of liquid to be tested.
- If testing more than one liquid, label each test tube with a marker.
- Add 3 drops of Biuret reagent solution to each test tube. Shake gently to mix.
- Note any color change. Proteins will turn solution pink or purple.
Explanation:
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Answered by
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Following steps are used to test if a food item contains protein:
1) Take a small quantity of the food items to be tested.
2) Grind/mash the food item.
3) Put the food item into a test tube.
4) Add 10 drops of water and shake the test tube.
5) Add two drops of copper sulphate solution to the test tube, using a dropper.
6) Add 10 drops of caustic soda solution using a dropper and shake the test tube.
7) Let the test tube rest in a test tube stand for a few minutes.
8) Observe the colour of the mixture.
9) Violet colour indicates the presence of proteins in the tested food item.
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