Question

write the protocol of isolation of protoplast?​

Answer 1

Protocol 1: - Remove the main vascular bundles from the leaves of lamb's lettuce and cut them into pieces if the leaves are large; - Transfer immediately these pieces into a Petri dish (or a bottle) containing 15 ml of digestion solution 1; - Incubate at 26°C in the dark and under gentle shaking (50 rpm); - After 10, ...

Answer 2

Answer:

Protocol 1: - Remove the main vascular bundles from the leaves of lamb's lettuce and cut them into pieces if the leaves are large; - Transfer immediately these pieces into a Petri dish (or a bottle) containing 15 ml of digestion solution 1; - Incubate at 26°C in the dark and under gentle shaking (50 rpm); - After 10, ...

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