Write the sequence for preparing temporary mount to show onion peel cells
Answers
1. Take an onion and remove its outermost peel.
2. Now cut a small part from an inner scale leaf with the help of a blade.
3. Separate a thin, transparent peel from the convex surface of the scale leaf with the help of forceps.
4. Keep this peel in a watch glass containing water?
5. Add two drops of safranin stain in the watch glass to stain the peel.
6. Take a clean slide and put a drop of glycerine in the centre of the slide.
7. With the help of a brush and needle transfer the peel on the slide. Glycerine prevents the peel from drying up.
8. Carefully cover it with a coverslip and avoid any air bubble from entering interring the coverslip.
9. Remove any excessive glycerine with a filter paper.
10. Observe the prepared mount of the peel under the low and high magnification of a compound microscope.
Hope it helps :)