Question

Write the steps imvolved in making slide of epidermal slide of

Answer 1

Pluck a fresh leaf from a balsam plant.
Fold the leaf and carefully tear along the bruised area of the lower side of the leaf.
We can see a colourless narrow border along the torn edge.
Carefully pull out the thin membranous transparent layer from the lower epidermis using a forceps.
Put the epidermis into a watch glass containing distilled water.
Take few drops of Safranin solution using a dropper and transfer this into another watch glass.
Using a brush transfer the epidermis into the watch glass containing the Safranin solution.
Keep the epidermis for 30 sec in the Safranin solution to stain the peel.
To remove excess stain sticking to the peel, place it again in the watch glass containing water.
Place the peel onto a clean glass slide using the brush.
Take a few drops of glycerine using a dropper and pour this on the peel.
Using a needle, place a cover slip over the epidermis gently.
Drain out the excess glycerine using a blotting paper.
Take the glass slide and place it on the stage of the compound microscope.
Examine the slide through the lens of the compound microscope.