Question

Write the steps in restriction mapping. Mention use of this technique.

Answer 1

Restriction mapping is a very useful technique when used for determining the orientation of an insert in a cloning vector, by mapping the position of an off-center restriction site in the insert.

Answer 2

ÊLLØ'!!

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•Restriction mapping is a physical mapping technique which is used to determine the relative location of restriction sites on a DNA fragment to give a restriction map.

•Restriction enzymes are endonucleases that recognize specific sequences on DNA and make specific cuts.

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