Write the steps involved in preparing temporary mount of leaf peel to show stomata
Answers
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1. Remove a healthy leaf from the potted plant.
2. Remove a part of the peel from the lower surface of the leaf. You can do this by folding the leaf over and gently pulling the peel apart using forceps. Keeps the peel in a watch glass containing water.
3. Put a few drops of safranin stain in a watch glass.
4. After 2-3 minutes take out the peel and place it on a clean glass slide.
5. Put a drop of glycerin over the peel and place a clean coverslip gently over it with the help of a needle.
6. Remove the excess stain and glycerin with the help of blotting paper.
7. Observe the slide under the low-power and high-power magnifications of the compound microscope.
I hope you have understood.
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Step 1. A lily leaf is taken and simply fold in the centre which gets broken into two pieces.
Step 2. one leaf piece is gently pulled and we got a transparent leaf peel.
Step 3 . The leaf peel of lower epidermis is taken and cut with scissors into small pieces. These pieces transferred with the help of paintbrush into a petri dish containing water.
Step 4. A piece of leaf is selected and transferred to another watch glass containing dilute solution of sadhanon.
Step 5. With the help of paintbrush specimen is transferred back into the first water containing petrified.
Step 6. Specimen is transferred to slide with the help of brush.
Step 7. A drip of glycerin is put on the peel and it is covered carefully with a cover slip by using needle to avoid air bubbles . Excess glycerin is removed with the help of filter paper.
Step 8. The leaf peel is observed under low power of microscope.