You are in a laboratory attempting to identify a genetic defect responsible for a disease. You believe you have located the gene that results in a faulty protein—but you aren't sure! How could you be sure you have located a section of DNA that encodes for a protein? Write down the sequence to see what amino acids might be linked together. Allow the DNA to be transcribed to RNA and see what protein results. Compare the faulty protein to the DNA. Building Proteins
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Using PCR you can find the gene sequences and then corresponding amino acid can also found. Otherwise such DNA can be attached to vector and replicated in a bacteria and multiply then in nutrient media. Separate protein after multiplication and identify proteins.
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Allow the DNA to be transcribed to RNA and see what protein results.
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