You are provided with the three solutions:A ,B and C . one solution contains the enzyme amylase, one contains starch and one contains glucose. Starch is the substrate of the enzyme. The product is the sugar maltose. You are provided with only one reagent, Benedict's solution, and the usual laboratory apparatus.
A => outline the procedure you would follow to identify the three solutions
Answers
Answer: (a)carry out Benedict’s test on solutions A, B and C;
a positive result/brick-red precipitate will be seen, with the glucose solution;
heat separate samples of the two remaining solutions, in boiling water bath/to high
temperature (e.g. 80 °C), for suitable time/at least two minutes (enzyme will be denatured);
for each heated solution, mix it with an unheated sample of the other solution;
leave several minutes/suitable time (for reaction to take place);
carry out Benedict’s test on the two tubes;
only one will give a positive result (due to presence of maltose) and this will be the one
which contained the unheated enzyme;
accept alternative wording for all steps in the procedure, provided the same logical sequence
is described [max. 6]
(b) hydrolysis
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