you are working in a Culture Lab your senior given in little volume of sample transformed cells for large scale production of Recombinant product how will you achieve the target given
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After having cloned the gene of interest and having optimised the conditions to induce the expression of the target protein one has to consider producing it on a large scale. Small volume cultures cannot yield appreciable quantities of products.
To produce in large quantities the development of bioreactors, where large volume (100-1000 litres) of culture (continuous) can be processed, was required.
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