( you can ligate a foreign DNA
at the BamH I site of tetracycline
resistance gene in the vector PBR322.
The recombinant plasmids will lose
tetracycline resistance due to insertion
of foreign DNA but can still be selected
out from non-recombinant ones by
plating the transformants on ampicillin
containing medium. )
please explanation simply
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you can ligate a foreign DNA
at the BamH I site of tetracycline
resistance gene in the vector PBR322.
The recombinant plasmids will lose
tetracycline resistance due to insertion
of foreign DNA but can still be selected
out from non-recombinant ones by
plating the transformants on ampicillin
containing medium. )
please explanation simply
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