Question

you do a kinetic experiment and calculate
a Vmax of 100µmol of product per minute. If each assay used 0.1ml of an enzyme solution that had a protein concentration of 0.2mg/ml,what would be the turnover number, if the enzyme had a molecular weight of 128,000g/mol?

Answer 1

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Answer 2

Answer:

Explanation:

One Unit is defined as the amount of the enzyme that produces a certain amount of enzymatic activity, that is, the amount that catalyzes the conversion of 1 micro mole of substrate per minute. A spectrophotometric assay is usually applied for this purpose by a selected substrate. Enzyme activity could be calculated using the following equation:

activity (U/L) = ((absorbance variation/time)/molar extintion coefficent * path length) * 1 micromol * (total reaction volume/total enzyme volume)

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