Question

You have one more veggie lysate, beets, that you need to analyze. However the absorbance of beet protein lysate couldn’t be recorded. Seems it above the upper threshold for the spectrophotometer. You really need to figure out the concentration, so you decide to make a couple beet dilutions, record absorabces of those dilutions.

(a) The first dilution you prep is DF =20. The absorbance of the diluted (1:20) beet lysate = 0.9. What is the concentration of this (1:20) beet lysate? Now figure out what the concentration of the un-diluted beet lysate is (refer back to question #3 if you need help)

(b) You also made a beet lysate dilution at 1:100. The absorbance of this (1:100) diluted beet lysate = 0.04; what is the concentration of the 1:100 beet lysate? What about of the undiluted beet lysate?

(c) You also made a beet lysate dilution at 1:50. The absorbance of this (1:50) diluted beet lysate = 0.1; what is the concentration of the 1:50 beet lysate? What about of the undiluted beet lysate?

(d) Were your answers for undiluted beet lysate concentrations (in a-c) the same or different? If they were different, you need to reconcile for this difference by taking the average of these values. What do you conclude is the concentration of the undiluted beet sample?

Answer 1

Answer:

(c) You also made a beet lysate dilution at 1:50. The absorbance of this (1:50) diluted beet lysate = 0.1; what is the concentration of the 1:50 beet lysate? What about of the undiluted beet lysate