z list out the apparatus (materials and chemicals)
you used in the observations of root tip
experiment? explain brefly.
Answers
Answer:
Aim Of The Experiment
To study and demonstrate mitosis by preparing the mount of an onion root tip cells.
Theory Of The Experiment
For entities to mature, grow, maintain tissues, repair and synthesize new cells, cell division is required. Cell division is of two types:
Mitosis
Meiosis
Mitosis
In mitosis, the nucleus of the Eukaryotic cells divides into two, subsequently resulting in the splitting of the parent cells into two daughter cells. Hence, every cell division involves two chief stages:
Cytokinesis – Cytoplasm division
Karyokinesis – Nucleus division
Stages Of Mitosis
The various stages of mitosis are:
1. Prophase
The process of mitosis is initiated at this stage wherein coiling and thickening of the chromosomes occurs
Shrinking and hence the disappearance of the nucleolus and nuclear membrane takes place
The stage reaches its final state when a cluster of fibres organizes to form the spindle fibres.
2. Metaphase
Chromosomes turn thick in this phase. The two chromatids from each of the chromosomes appear distinct
Each of the chromosomes is fastened to the spindle fibres located on its controller
Chromosomes align at the centreline of the cell
3. Anaphase
Each of the chromatid pair detaches from the centromere and approaches the other end of the cell through the spindle fibre
At this stage, compressing of the cell membrane at the centre takes place
4. Telophase
Chromatids have reached the other end of the cell
The disappearance of the spindles
Chromatin fibres are formed as a result of uncoiling of daughter chromosomes
The appearance of two daughter nuclei at the opposing ends due to the reformation of the nucleolus and nuclear membrane
At this phase, splitting of the cell or cytokinesis may also occur.
Post mitosis, the next stage is referred to as interphase which is part of the cell cycle that is non-dividing and between two consecutive cell divisions. A cell spends most of its life in the interphase. It comprises the G1, S and G2 stages.
Answer:
microscope is use for the apparatus ( materials and chemicals) you need in the observations of the root tip experiments.