1)Trypsin is used for dissociating the tissue into single cells.
a) True
Ans. A
2)In the secondary culture, cells are obtained from _______________________
a) primary culture
Ans. A
3)EDTA binds the ______________ ions.
d) calcium
Ans. D
4) HeLa cells are a cell line.
a) True
Ans. A
5) Under favorable conditions, the protoplasts can grow into a ____________________
a) callus culture
Ans. A
6) The process of dedifferentiation in cell culture can give rise to ________________________
a) induced-pluripotent stem cells
Ans. A
7)Isolation of an organelle in bulk quantity can be accomplished by _____________
c) differential centrifugation
Ans.c
8)The isotonic buffer solution prevents _________
d) membrane rupture
Ans. D
9)Density gradient centrifugation is a type of isopycnic centrifugation.
a) True
Ans. A
10)Which of the following can be used to study cellular activities?
b) Cell-free systems
Ans.b
11. Who is known as the Father of tissue culture?
Sol: (c) Haberlandt.
12. The production of secondary metabolites requires the use of ________.
Sol:(d) Cell suspension.
13. The pair of hormones required for a callus to differentiate are________.
Sol: (b) Auxin and cytokinin.
14. What is Dimethyl sulfoxide used for?
Sol: (b) Cryoprotectant.
15. The formation of embryoids from the pollen grains in the tissue culture medium is due to ________.
Sol: (d) Cellular totipotency.
16. Synthetic seeds are produced by the encapsulation of somatic embryos with___________.
Sol: (d) Sodium alginate.
17. Totipotency refers to ___________.
Sol: (b) Development of an organ from a cell in a culture medium.
18. Which of the following is the main application of embryo culture?
Sol: (d) Overcoming hybridisation barriers.
19. In tissue culture of
parenchyma, mitosis is accelerated in the presence of ________.
Sol: (d) Both auxin and cytokinin.
20. In which of the following conditions do the somaclonal variations appear?
.
Sol: (a) Plants raised in tissue culture.
21. Haploid plants can be obtained from________.
Sol: (a) Anther culture.
22. In-plant tissue culture, the callus tissues are generated into a complete plantlet by altering the concentration________.
Sol: (b) Hormones.
23. Which of the following is cultured to obtain haploid plants?
Sol: (d) Entire anther.
24. Which of the following vectors is used in crop improvement and crop management?
Sol: (a) Agrobacterium.
25. Which of the following growth hormones produces apical dominance?
Sol: (c) Auxin.
26. Cybrids are produced by
Sol: (a) Nucleus of one species but cytoplasm from both the parent species.
27. Which of the following mediums is composed of chemically defined compounds?
Sol: (c) Synthetic media.
28. Which of the following chemicals are most widely used for protoplast fusion?
Sol: (b) Polyethylene glycol.
29. Which of the following plant cells shows totipotency?
Sol: (b) Meristem.
30. What is Callus?
Sol: (b) An unorganised actively dividing mass of cells maintained in culture.
Answers
1)Trypsin is used for dissociating the tissue into single cells.
a) True
Ans. A
2)In the secondary culture, cells are obtained from _______________________
a) primary culture
Ans. A
3)EDTA binds the ______________ ions.
d) calcium
Ans. D
4) HeLa cells are a cell line.
a) True
Ans. A
5) Under favorable conditions, the protoplasts can grow into a ____________________
a) callus culture
Ans. A
6) The process of dedifferentiation in cell culture can give rise to ________________________
a) induced-pluripotent stem cells
Ans. A
7)Isolation of an organelle in bulk quantity can be accomplished by _____________
c) differential centrifugation
Ans.c
8)The isotonic buffer solution prevents _________
d) membrane rupture
Ans. D
9)Density gradient centrifugation is a type of isopycnic centrifugation.
a) True
Ans. A
10)Which of the following can be used to study cellular activities?
b) Cell-free systems
Ans.b
11. Who is known as the Father of tissue culture?
Sol: (c) Haberlandt.
12. The production of secondary metabolites requires the use of ________.
Sol:(d) Cell suspension.
13. The pair of hormones required for a callus to differentiate are________.
Sol: (b) Auxin and cytokinin.
14. What is Dimethyl sulfoxide used for?
Sol: (b) Cryoprotectant.
15. The formation of embryoids from the pollen grains in the tissue culture medium is due to ________.
Sol: (d) Cellular totipotency.
16. Synthetic seeds are produced by the encapsulation of somatic embryos with___________.
Sol: (d) Sodium alginate.
17. Totipotency refers to ___________.
Sol: (b) Development of an organ from a cell in a culture medium.
18. Which of the following is the main application of embryo culture?
Sol: (d) Overcoming hybridisation barriers.
19. In tissue culture of
parenchyma, mitosis is accelerated in the presence of ________.
Sol: (d) Both auxin and cytokinin.
20. In which of the following conditions do the somaclonal variations appear?
.
Sol: (a) Plants raised in tissue culture.
21. Haploid plants can be obtained from________.
Sol: (a) Anther culture.
22. In-plant tissue culture, the callus tissues are generated into a complete plantlet by altering the concentration________.
Sol: (b) Hormones.
23. Which of the following is cultured to obtain haploid plants?
Sol: (d) Entire anther.
24. Which of the following vectors is used in crop improvement and crop management?
Sol: (a) Agrobacterium.
25. Which of the following growth hormones produces apical dominance?
Sol: (c) Auxin.
26. Cybrids are produced by
Sol: (a) Nucleus of one species but cytoplasm from both the parent species.
27. Which of the following mediums is composed of chemically defined compounds?
Sol: (c) Synthetic media.
28. Which of the following chemicals are most widely used for protoplast fusion?
Sol: (b) Polyethylene glycol.
29. Which of the following plant cells shows totipotency?
Sol: (b) Meristem.
30. What is Callus?
Sol: (b) An unorganised actively dividing mass of cells maintained in culture.