Question

11. Bacterial gene delivery is known as
(A) Transduction
(B) Bactofection
(C) Infection
(D) Conjugation
12. Which of the following do not incorporate in the genome and replicates in the cytoplasm?
(A) Poxvirus
(B) Baculovirus
(C) Adenovirus
(D) Retrovirus
13. Which of the following does not act as a restriction enzyme?
(A) Ecorl
(B) BamHI
(C) Hind III
(D) polydeoxyribonucleotide synthase
14. Which of the following enzyme is responsible for making a DNA copy from RNA?
(A) Reverse transcriptase
(B) DNA polymerase
(C) RNA poll
(D) RNA polli
15. Which of the following can be termed as a restriction modification system?
(A) Restriction endonuclease + methylase
(B) DNA ligase + methylase
(C) Restriction endonuclease + acetylase
(D) DNA ligase + acetylase
16. Which of the following is true about restriction endonucleases?
(A) Type I and II requires ATP to move along DNA
(B) Type I, II and III requires ATP to move along DNA
(C) Type II requires no ATP and cleaves DNA within recognition sequence
(D) Type II requires ATP and cleaves DNA within recognition sequence
17. Which of the following enzymes' combined action leads to the generation of sticky ends in the plasmid
vector?
(A) Alkaline phosphatase and terminal transferase
(B) Exonuclease III and alkaline phosphatase
(C) Bacteriophage lambda exonuclease and terminal transferase
(D) Exonuclease III and terminal transferase
18. Which of the following enzyme is required for end to end joining of DNA?)
(A) DNA ligase
(B) Restriction endonuclease
(C) RNA polymerase
(D) DNA polymeras
19. Chain-termination is a type of
(A) Sequencing
(B) Vector generation
(C) Antibiotic production
(D) Gene manipulation
20. The first significant DNA sequence to be obtained was that of

Answer 1

Answer:

1. (B) Bactofection
3. (D) polydeoxyribonucleotide synthase
4. (A) Reverse transcriptase
5. (A) Restriction endonuclease + methylase
6. (C) Type II requires no ATP and cleaves DNA within recognition sequence
7. (C) Bacteriophage lambda exonuclease and terminal transferase
8. (A) DNA ligase
9. (A) Sequencing

                      Hope it helps you^_^

Answer 2

Answer:

11. (B)

• Bactofection is a powerful method for expressing plasmid-encoded heterologous proteins (protein antigens, toxins, or enzymes) in a variety of cell types, including phagocytic and nonphagocytic mammalian cells. Bacteria are used to transmit plasmid DNA into mammalian cells.

12. (A)

• The majority of double-stranded DNA viruses, such as poxviruses, polyomaviruses, adenoviruses, and herpesviruses, replicate in the nucleus of the host cell.
• Herpes and adenoviruses both encode their own replication components.

13. (D)

• Polydeoxyribonucleotide synthase is a DNA ligase.

14. (A)

• An enzyme found in the genetic material of retroviruses termed reverse transcriptase, also known as RNA-directed DNA polymerase, is responsible for facilitating the transcription of retrovirus RNA (ribonucleic acid) into DNA (deoxyribonucleic acid).

15. (A)

• A defense mechanism against foreign DNA, such as that carried by bacteriophages, is the restriction modification system (RM system), which is present in bacteria and other prokaryotic organisms.

16. (C)

• There are four categories of restriction enzymes—I, II, III, and IV—that have historically been recognized. These categories differ mainly in structure, cleavage site, specificity, and cofactors.

17. (C)

• The plasmid vector develops sticky ends as a result of the combined action of the bacteriophage lambda exonuclease and terminal transferase.

18. (A)

• A DNA-joining enzyme is called DNA ligase. Ligase can join two sections of DNA that have identical ends together to create a single, uninterrupted DNA molecule.

19. (A)

• The "chain termination method," often known as Sanger sequencing, is a technique for figuring out the nucleotide sequence of DNA.

20. Cohesive ends of lambda, which were 12 bases long, were the first notable DNA to be discovered in 1971.

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