Question

18. Significance of treating bacterial cells with calcium chloride before transformation is to facilitate (1) Binding of DNA to the cell surface (2) Uptake of DNA through membrane transport proteins (3) Uptake of DNA by creating transient pores in the bacterial cell wall (4) Expression of antibiotic resistance gene
acc to me answer should be 3 but it is 1 can anyone explain​

Answer 1

Answer:

A unicellular organism, also known as a single-celled organism, is an organism that consists of a single cell, unlike a multicellular organism that consists of multiple cells. Unicellular organisms fall into two general categories: prokaryotic organisms and eukaryotic organisms

Answer 2

Answer:

Gene transfer using calcium chloride is known as heat shock method. In this method, the cells in which DNA has to be transferred i.e. host cells or bacterial cells, are incubated with the vector in a solution containing calcium ions at a low temperature. The temperature is then suddenly raised and this heat shock causes the cells to take up the vector because the sudden heat develops pores in the bacterial cell wall or membranes..

So, the correct answer is 'Uptake of DNA by creating transient pores in the bacterial cell wall'.