2 vital conditions for artificial transformation
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Transformation is one of three processes for horizontal gene transfer, in which exogenous genetic material passes from one bacterium to another, the other two being conjugation(transfer of genetic material between two bacterial cells in direct contact) and transduction (injection of foreign DNA by a bacteriophage virus into the host bacterium).In transformation, the genetic material passes through the intervening medium, and uptake is completely dependent on the recipient bacterium.
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