Question

2. Which of the following is a mismatch?
a) Polymerase – Taq polymerase
b) Template – double stranded DNA
C) Primer - oligonucleotide
d) Synthesis - 5' to 3' direction

Answer 1

Answer:

b) Template - double-stranded DNA

Explanation:

1. Polymerase - Taq polymerase - It is correct. Taq polymerase is a type of polymerase which is obtained from Thermus aquaticus for the replication of DNA strands in the PCR reaction.
2. Template - double-stranded DNA - It is a mismatch. A template is a single strand of DNA which is also known as a non-coding strand. Transcription occurs on this strand and forms an RNA strand which is complementary to this template strand.
3. Primer - Oligonucleotide - It is correct. Primer is involved in the replication and is made up of 13-25 nucleotides. These hybridize specifically to DNA or RNA sequences.
4. Synthesis - 5’ to 3’ direction - It is correct. Synthesis of an RNA strand in the transcription occurs in the direction 5’ to 3’ direction on a 3’ to 5’ template strand.

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