Question

4. What issue came about during the cloning of miRNA-433?​

Answer 1

Explanation:

miRNAs (microRNAs) are short non-coding RNAs that regulate gene expression post-transcriptionally. They generally bind to the 3'-UTR (untranslated region) of their target mRNAs and repress protein production by destabilizing the mRNA and translational silencing.

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Answer 2

Answer:

ᴀᴘᴘʟɪᴇᴅ ʙɪᴏsʏsᴛᴇᴍs ᴛᴀǫᴍᴀɴ ᴀᴅᴠᴀɴᴄᴇᴅ ᴍɪʀɴᴀ ᴀssᴀʏs ᴇɴᴀʙʟᴇ ʜɪɢʜʟʏ sᴇɴsɪᴛɪᴠᴇ ᴀɴᴅ sᴘᴇᴄɪғɪᴄ ǫᴜᴀɴᴛɪғɪᴄᴀᴛɪᴏɴ ᴏғ ᴍᴀᴛᴜʀᴇ ᴍɪʀɴᴀs ᴜsɪɴɢ ǫᴘᴄʀ. ᴛᴏɢᴇᴛʜᴇʀ ᴡɪᴛʜ ᴛʜᴇ ᴛᴀǫᴍᴀɴ ᴀᴅᴠᴀɴᴄᴇᴅ ᴍɪʀɴᴀ ᴄᴅɴᴀ sʏɴᴛʜᴇsɪs ᴋɪᴛ, ᴛʜɪs sᴏʟᴜᴛɪᴏɴ ɪs ᴅᴇsɪɢɴᴇᴅ ᴛᴏ ᴘʀᴏᴠɪᴅᴇ ᴀ sᴛʀᴇᴀᴍʟɪɴᴇᴅ ᴡᴏʀᴋғʟᴏᴡ ᴛʜᴀᴛ ᴀʟʟᴏᴡs ғᴏʀ ᴇxᴄᴇᴘᴛɪᴏɴᴀʟ ᴅᴇᴛᴇᴄᴛɪᴏɴ ᴏғ ᴍᴜʟᴛɪᴘʟᴇ ᴍɪʀɴᴀ ᴛᴀʀɢᴇᴛs ғʀᴏᴍ ᴀ sɪɴɢʟᴇ sᴀᴍᴘʟᴇ.

hope it helps uhh

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